Plasmid

Part:BBa_K4354010:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-29)


plasmid pHY-P43


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 333
    Illegal BglII site found at 307
    Illegal BamHI site found at 323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
    Illegal AgeI site found at 5005
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2883
    Illegal BsaI site found at 4300


Design Notes

We plan to remove the Ampicillin resistance gene because of the incompatibility between plasmid pHY-P43 and pUC19.


Source

We bought it from HonorGene company.

References